RETRACTED: RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA | Zendy

Yalei Cui | Zendy; Tianzhi Huang | Zendy; Xiaobo Zhang | Zendy

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RETRACTED: RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA

Author(s) -

Yalei Cui,

Tianzhi Huang,

Xiaobo Zhang

Publication year - 2015

Publication title -

open biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.078

H-Index - 53

ISSN - 2046-2441

DOI - 10.1098/rsob.150126

Subject(s) - biology , rna editing , rna silencing , gene silencing , microrna , adar , rna , rna interference , white spot syndrome , genetics , rna induced silencing complex , messenger rna , trans acting sirna , gene , computational biology

MicroRNAs (miRNAs) integrate with Argonaut (Ago) to create the RNA-induced silencing complex, and regulate gene expression by silencing target mRNAs. RNA editing of miRNA may affect miRNA processing, assembly of the Ago complex and target mRNA binding. However, the function of edited miRNA, assembled within the Ago complex, has not been extensively investigated. In this study, sequence analysis of the Ago complex of Marsupenaeus japonicus shrimp infected with white spot syndrome virus (WSSV) revealed that host ADAR (adenosine deaminase acting on RNA) catalysed A-to-I RNA editing of a viral miRNA (WSSV-miR-N12) at the +16 site. This editing of the non-seed sequence did not affect association of the edited miRNA with the Ago protein, but inhibited interaction between the miRNA and its target gene ( wsv399 ). The WSSV early gene wsv399 inhibited WSSV infection. As a result, the RNA editing of miRNA caused virus latency. Our results highlight a novel example of miRNA editing in the miRNA-induced silencing complex.

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