Evaluation of burst release and sustained release of pioglitazone-loaded fibrous mats on diabetic wound healing: an in vitro and in vivo comparison study | Zendy

Muhammet Emin Çam | Zendy; Sila Yildiz | Zendy; Hussain Alenezi | Zendy; Sumeyye Cesur | Zendy; Gul Sinemcan Ozcan | Zendy; Gökçe Erdemir | Zendy; Ursula Edirisinghe | Zendy; Dilek Akakın | Zendy; Serap Erdem Kuruca | Zendy; Levent Kabasakal | Zendy; Oğuzhan Gündüz | Zendy; Mohan Edirisinghe | Zendy

Open Access

Evaluation of burst release and sustained release of pioglitazone-loaded fibrous mats on diabetic wound healing: an in vitro and in vivo comparison study

Author(s) -

Muhammet Emin Çam,

Sila Yildiz,

Hussain Alenezi,

Sumeyye Cesur,

Gul Sinemcan Ozcan,

Gökçe Erdemir,

Ursula Edirisinghe,

Dilek Akakın,

Serap Erdem Kuruca,

Levent Kabasakal,

Oğuzhan Gündüz,

Mohan Edirisinghe

Publication year - 2020

Publication title -

journal of the royal society interface

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.655

H-Index - 139

eISSN - 1742-5689

pISSN - 1742-5662

DOI - 10.1098/rsif.2019.0712

Subject(s) - wound healing , in vivo , pioglitazone , pharmacology , chemistry , fibroblast , agonist , inflammation , biomedical engineering , in vitro , receptor , medicine , diabetes mellitus , surgery , endocrinology , type 2 diabetes , biology , biochemistry , microbiology and biotechnology

In order to provide more effective treatment strategies for the rapid healing of diabetic wounds, novel therapeutic approaches need to be developed. The therapeutic potential of peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist pioglitazone hydrochloride (PHR) in two different release kinetic scenarios, burst release and sustained release, was investigated and compared within vitro andin vivo tests as potential wound healing dressings. PHR-loaded fibrous mats were successfully fabricated using polyvinyl-pyrrolidone and polycaprolactone by scalable pressurized gyration. The results indicated that PHR-loaded fibrous mats expedited diabetic wound healing in type-1 diabetic rats and did not show any cytotoxic effect on NIH/3T3 (mouse embryo fibroblast) cells, albeit with different release kinetics and efficacies. The wound healing effects of fibrous mats are presented with histological and biochemical evaluations. PHR-loaded fibrous mats improved neutrophil infiltration, oedema, and inflammation and increased epidermal regeneration and fibroblast proliferation, but the formation of hair follicles and completely improved oedema were observed only in the sustained release form. Thus, topical administration of PPAR-γ agonist in sustained release form has high potential for the treatment of diabetic wounds in inflammatory and proliferative phases of healing with high bioavailability and fewer systemic side effects.

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