Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting

We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section. Furthermore, the scattered-light-induced background is significantly reduced, considerably improving image contrast. Our method is expected to expand the application field of LSFM into the optical quasi-ballistic regime, enabling studies on non-transparent biological samples.