Open AccessNuclear magnetic resonance analysis of protein–DNA interactions
Author(s) -
Sébastien Campagne,
Virginie Gervais,
Alain Milon
Publication year - 2011
Publication title -
journal of the royal society interface
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 139
eISSN - 1742-5689
pISSN - 1742-5662
DOI - 10.1098/rsif.2010.0543
Subject(s) - computational biology , structural biology , dna , computer science , nanotechnology , physics , data science , biology , genetics , materials science
Recent methodological and instrumental advances in solution-state nuclear magnetic resonance have opened up the way to investigating challenging problems in structural biology such as large macromolecular complexes. This review focuses on the experimental strategies currently employed to solve structures of protein–DNA complexes and to analyse their dynamics. It highlights how these approaches can help in understanding detailed molecular mechanisms of target recognition.