Monitoring yeast mitochondria with peroxiredoxin-based redox probes: the influence of oxygen and glucose availability | Zendy

Daniel PastorFlores | Zendy; Katja Becker | Zendy; Tobias P. Dick | Zendy

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Monitoring yeast mitochondria with peroxiredoxin-based redox probes: the influence of oxygen and glucose availability

Author(s) -

Daniel PastorFlores,

Katja Becker,

Tobias P. Dick

Publication year - 2017

Publication title -

interface focus

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 49

eISSN - 2042-8901

pISSN - 2042-8898

DOI - 10.1098/rsfs.2016.0143

Subject(s) - peroxiredoxin , pentose phosphate pathway , redox , mitochondrion , reactive oxygen species , biochemistry , oxidative phosphorylation , cytosol , chemistry , yeast , microbiology and biotechnology , mitochondrial matrix , oxygen , biology , glycolysis , metabolism , enzyme , peroxidase , organic chemistry

Mitochondrially generated oxidants are believed to play important roles in both physiology and pathophysiology. Therefore, it is of significant interest to better understand the metabolic conditions leading to enhanced mitochondrial oxidant generation. Here, we investigate the influence of oxygen and glucose availability on the redox state of peroxiredoxin-based redox probes, expressed in the cytosol and mitochondrial matrix of yeast cells. We observe that the redox state of peroxiredoxin probes reflects the balance between dioxygen-dependent peroxide generation and glucose-dependent generation of reducing equivalents. The oxidative pentose phosphate pathway appears to be the dominant source of NADPH in the system under study.

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