A cell-free approach to accelerate the study of protein–protein interactions in vitro | Zendy

Emma Sierecki | Zendy; Nichole Giles | Zendy; Mark Polinkovsky | Zendy; Mehdi Moustaqil | Zendy; Kirill Alexandrov | Zendy; Yann Gambin | Zendy

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A cell-free approach to accelerate the study of protein–protein interactions in vitro

Author(s) -

Emma Sierecki,

Nichole Giles,

Mark Polinkovsky,

Mehdi Moustaqil,

Kirill Alexandrov,

Yann Gambin

Publication year - 2013

Publication title -

interface focus

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 49

eISSN - 2042-8901

pISSN - 2042-8898

DOI - 10.1098/rsfs.2013.0018

Subject(s) - computer science , computational biology , protein–protein interaction , bioinformatics , chemistry , biology , biochemistry

Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.

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