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An increase in foliar nitrogen isotope composition (δ15 N) with decreasing precipitation has been shown to occur widely in non-N2 -fixing plant species. However, similar patterns have not been identified in N2 -fixing species. Here, we tested the relationships of foliar δ15 N with local environmental factors and leaf properties in two leguminous shrub species (Caragana korshinskii andCaragana liouana ) sampled from 30 populations. Results indicated that the mean annual precipitation (MAP) primarily accounted for the variation of foliar δ15 N in the two species. Further analysis revealed strong negative correlations between foliar δ15 N and MAP within and across species. These results suggest that the foliar δ15 N of leguminous shrub species also shift along precipitation gradients, which augments our understanding of the relationships between foliar δ15 N and climatic factors.

Precipitation is the main factor affecting the variation of foliar nitrogen isotope composition in two leguminous shrub species of northwestern China