Pouch brooding marsupial frogs transfer nutrients to developing embryos

Marsupial frogs have a unique reproductive mode in which females carry eggs enclosed in a sealed dorsal brood pouch. While most anurans are considered to be oviparous with lecithotrophic eggs, the extensively vascularized membrane of the brood pouch in marsupial frogs suggests potential opportunities for nutrient transfer. We tested for matrotrophy in the live-bearingGastrotheca excubitor (Hemiphractidae), through feeding insects labelled with a13 C-fatty acid and a15 N-amino acid to brooding marsupial frogs. We observed significant increases of δ13 C and δ15 N in both maternal pouch tissues and embryos, suggesting nutrient transfer. Embryo dry mass also increased with developmental stage, providing further direct evidence for matrotrophy. These results suggest that in addition to gas exchange, the vascularized brood pouch membrane ofG. excubitor also enables maternal nutrient transfer. This finding revealed a suspected but untested trait in the evolution of parental care in marsupial frogs, in contrast to previous work onGastrotheca species that release tadpoles, and suggests greater complexity in reproductive and provisioning modes than previously thought.