Taqman real-time quantitative PCR for identification of western flower thrip ( Frankliniella occidentalis ) for plant quarantine | Zendy

Kai Huang | Zendy; S. E. Lee | Zendy; Y. Yeh | Zendy; G. Shen | Zendy; Elena Mei | Zendy; Chung-Ming Chang | Zendy

Open Access

Taqman real-time quantitative PCR for identification of western flower thrip ( Frankliniella occidentalis ) for plant quarantine

Author(s) -

Kai Huang,

S. E. Lee,

Y. Yeh,

G. Shen,

Elena Mei,

Chung-Ming Chang

Publication year - 2010

Publication title -

biology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.596

H-Index - 110

eISSN - 1744-957X

pISSN - 1744-9561

DOI - 10.1098/rsbl.2009.1060

Subject(s) - biology , taqman , quarantine , identification (biology) , real time polymerase chain reaction , plant quarantine , botany , genetics , ecology , gene

Western flower thrip (Frankliniella occidentalis) is a major global pest of agricultural products. It directly damages crops through feeding, oviposition activity or transmission of several plant viruses. We describe a Taqman real-time quantitative PCR detection system, which can rapidly identify F. occidentalis from thrips larvae to complement the traditional morphological identification. The data showed that our detection system targeted on the ribosomal RNA gene regions of F. occidentalis has high sensitivity and specificity. The rapid method can be used for on-site testing of samples at ports-of-entry in the future.

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