Open AccessOptimal Cutoff Point for Immunoperoxidase Detection of C4d in the Renal Allograft: Results From a Multicenter Study
Author(s) -
Gretchen S. Crary,
Yassaman Raissian,
Robert Gaston,
Sita Gourishankar,
Robert Leduc,
Roslyn B. Man,
Arthur J. Matas,
Joseph P. Grande
Publication year - 2010
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0b013e3181f7fec9
Subject(s) - cutoff , peritubular capillaries , confidence interval , staining , medicine , hazard ratio , biopsy , urology , immunoperoxidase , pathology , gastroenterology , transplantation , immunology , monoclonal antibody , physics , antibody , quantum mechanics
Although C4d deposition in peritubular capillaries has been identified as a strong risk factor for subsequent renal allograft loss, the optimal cutoff for the fraction of peritubular capillaries needed to establish a positive stain in formalin-fixed, paraffin-embedded material has not been defined systematically. The objective of this study was to establish the threshold for positive staining that best predicts renal outcome in renal biopsies in a multicenter study in which local and central pathologic conditions were compared.