Open AccessDevelopment of a Humanized Mouse Model to Study the Role of Macrophages in Allograft Injury
Author(s) -
Nancy C. Kirkiles-Smith,
Mark J. Harding,
Benjamin R. Shepherd,
Stacey A. Fader,
Tai Yi,
Yig Wang,
Jennifer M. McNiff,
Edward L. Snyder,
Marc I. Lorber,
George Tellides,
Jordan S. Pober
Publication year - 2009
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0b013e318192e05d
Subject(s) - cd34 , stem cell , cd68 , adoptive cell transfer , haematopoiesis , macrophage , immunology , transplantation , humanized mouse , cd14 , monocyte , pathology , biology , medicine , t cell , microbiology and biotechnology , immune system , in vitro , immunohistochemistry , surgery , biochemistry
Nearly half of all infiltrating leukocytes in rejecting human allografts are macrophages, yet, in comparison with T cells, much less is known about the contribution of this cell type to rejection. Our laboratory has previously described models of rejection of human skin or artery grafts in immunodeficient mouse hosts mediated by adoptively transferred allogeneic T cells. However, mature human monocyte/macrophages have consistently failed to engraft in these animals. Here, we describe the introduction of human CD68+ macrophages into irradiated immunodeficient mice by transplantation of enriched CD34+ hematopoietic stem-cells isolated from peripheral blood of G-colony-stimulating factor pretreated adults.