Open AccessTargeting concatenated HIV antigens to human CD40 expands a broad repertoire of multifunctional CD4+ and CD8+ T cells
Author(s) -
Anne-Laure Flamar,
Yaming Xue,
Sandra Zurawski,
Monica Montes,
Bryan King,
Louis Sloan,
SangKon Oh,
Jacques Banchereau,
Yves Lévy,
Gérard Zurawski
Publication year - 2013
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/qad.0b013e3283624305
Subject(s) - cytotoxic t cell , biology , cd40 , antigen , antigen presenting cell , epitope , t cell , cd8 , interleukin 21 , major histocompatibility complex , dendritic cell , immunology , virology , immune system , in vitro , biochemistry
Targeting HIV antigens directly to dendritic cells using monoclonal antibodies against cell-surface receptors has been shown to evoke potent cellular immunity in animal models. The objective of this study was to configure an anti-human CD40 antibody fused to a string of five highly conserved CD4 and CD8 T-cell epitope-rich regions of HIV-1 Gag, Nef and Pol (αCD40.HIV5pep), and then to demonstrate the capacity of this candidate therapeutic vaccine to target these HIV peptide antigens to human dendritic cells to expand functional HIV-specific T cells.