Open AccessCoverage of primary mother-to-child HIV transmission isolates by second-generation broadly neutralizing antibodies
Author(s) -
Kyle J. Nakamura,
Chiara Cerini,
Edwin R. Sobrera,
Laura Heath,
Moses Sinkala,
Chipepo Kankasa,
Donald M. Thea,
James I. Mullins,
Louise Kuhn,
Grace M. Aldrovandi
Publication year - 2013
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/qad.0b013e32835cadd6
Subject(s) - neutralization , virology , antibody , neutralizing antibody , potency , transmission (telecommunications) , biology , virus , immunology , in vitro , genetics , computer science , telecommunications
A vaccine capable of providing cross-clade, sterilizing protection has been the holy grail of HIV-1 prevention and control since the beginning of the pandemic. A major component of this effort has been the identification and characterization of broadly neutralizing antibodies (bNAbs). Recent advances in bNAb isolation, structure-based engineering, and vector-mediated gene transfer have led to increased interest in bypassing the immune system by expressing neutralizing antibodies directly in muscle. To assess the neutralization potency and coverage of a panel of second-generation bNAbs, we cloned and phenotypically characterized 227 primary HIV-1 envelopes from 23 mother-to-child transmission (MTCT) pairs.