Open AccessFecal Microbiota Composition of Breast‐Fed Infants Is Correlated With Human Milk Oligosaccharides Consumed
Author(s) -
Wang Mei,
Li Min,
Wu Shuai,
Lebrilla Carlito B.,
Chapkin Robert S.,
Ivanov Ivan,
Donovan Sharon M.
Publication year - 2015
Publication title -
journal of pediatric gastroenterology and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 131
eISSN - 1536-4801
pISSN - 0277-2116
DOI - 10.1097/mpg.0000000000000752
Subject(s) - bacteroides , lachnospiraceae , feces , bifidobacterium , clostridium , enterobacter , infant formula , streptococcus , ruminococcus , breast milk , microbiology and biotechnology , microbiome , enterococcus , gut flora , bacteroidaceae , lactobacillus , veillonella , biology , medicine , food science , bacteria , immunology , 16s ribosomal rna , escherichia coli , antibiotics , biochemistry , bioinformatics , genetics , fermentation , gene , firmicutes
Objectives: This study tested the hypothesis that the fecal bacterial genera of breast‐fed (BF) and formula‐fed (FF) infants differ and that human milk oligosaccharides (HMOs) modulate the microbiota of BF infants. Methods: Fecal samples were obtained from BF (n = 16) or FF (n = 6) infants at 3‐month postpartum. Human milk samples were collected on the same day when feces were collected. The microbiota was assessed by pyrosequencing of bacterial 16S ribosomal RNA genes. HMOs were measured by high‐performance liquid chromatography–chip time‐of‐flight mass spectrometry. Results: The overall microbiota of BF differed from that of FF ( P = 0.005). Compared with FF, BF had higher relative abundances of Bacteroides , lower proportions of Clostridium XVIII, Lachnospiraceae incertae sedis , Streptococcus , Enterococcus , and Veillonella ( P < 0.05). Bifidobacterium predominated in both BF and FF infants, with no difference in abundance between the 2 groups. The most abundant HMOs were lacto‐N‐tetraose + lacto‐N‐neotetraose (LNT + LNnT, 22.6%), followed by 2′‐fucosyllactose (2′FL, 14.5%) and lacto‐N‐fucopentaose I (LNFP I, 9.5%). Partial least squares regression of HMO and microbiota showed several infant fecal bacterial genera could be predicted by their mothers' HMO profiles, and the important HMOs for the prediction of bacterial genera were identified by variable importance in the projection scores. Conclusions: These results strengthen the established relation between HMO and the infant microbiota and identify statistical means whereby infant bacterial genera can be predicted by milk HMO. Future studies are needed to validate these findings and determine whether the supplementation of formula with defined HMO could selectively modify the gut microbiota.