Open AccessMeasuring Protein Synthesis With SUnSET
Author(s) -
Craig A. Goodman,
Troy A. Hornberger
Publication year - 2013
Publication title -
exercise and sport sciences reviews
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 99
eISSN - 1538-3008
pISSN - 0091-6331
DOI - 10.1097/jes.0b013e3182798a95
Subject(s) - sunset , in vivo , protein biosynthesis , amino acid , biochemistry , chemistry , biophysics , biology , genetics , physics , astronomy
Protein synthesis rates commonly are measured using isotopic tracers to quantify the incorporation of a labeled amino acid into muscle proteins. Here we provide evidence supporting our hypothesis that the nonisotopic SUnSET technique is a valid and accurate method for the measurement of in vivo changes in protein synthesis at the whole-muscle and single-muscle fiber levels.