Open AccessA Human-Specific Mutation Limits Nonhuman Primate Efficacy in Preclinical Xenotransplantation Studies
Author(s) -
Joshua Waldman,
Linda G. Brock,
Michael Rees
Publication year - 2014
Publication title -
transplantation
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000441321.87915.82
Subject(s) - xenotransplantation , extracorporeal , hematocrit , perfusion , biology , immunology , nonhuman primate , medicine , pathology , transplantation , endocrinology , evolutionary biology
Patients diagnosed with fulminant hepatic failure face high mortality rates. A potential therapeutic approach for these patients is the use of extracorporeal porcine liver perfusion, to serve as a form of "liver dialysis." Previously, our laboratory has shown that, during a 72-hour extracorporeal perfusion with human blood, porcine Kupffer cells bind to and phagocytose human erythrocytes causing the hematocrit to fall to 2.5% of the original value. Subsequently, erythrocyte binding has been shown to involve N-acetylneuraminic acid (Neu5Ac) on the surface of human erythrocytes and sialoadhesin on the surface of the porcine Kupffer cells.