Open AccessEstablishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT
Author(s) -
Masanobu Maruyama,
Naoya Kobayashi,
Karen A. Westerman,
Masakiyo Sakaguchi,
Jean E. Allain,
Toshinori Totsugawa,
Teru Okitsu,
Takuya Fukazawa,
Anne Weber,
Donna B. Stolz,
Philippe Leboulch,
Noriaki Tanaka
Publication year - 2004
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000110292.73873.25
Subject(s) - cholangiocyte , telomerase reverse transcriptase , matrigel , transfection , telomerase , biology , cell culture , microbiology and biotechnology , viral vector , hek 293 cells , cancer research , gene , endocrinology , genetics , recombinant dna
Cholangiocytes perform an essential role in important pathophysiologic functions in the liver. Establishment of a human cholangiocyte line facilitates advances in cholangiocyte research and clinical applications for cell therapies. Here, we describe the immortalization of human cholangiocytes using serial transfection of simian virus 40 large T (SV40T) followed by human telomerase reverse transcriptase (hTERT).