Open AccessColitic scid mice fed Lactobacillus spp. show an ameliorated gut histopathology and an altered cytokine profile by local T cells
Author(s) -
Møller Peter Lange,
Pærregaard Anders,
Gad Monika,
Kristensen Nany,
Claesson Mogens Helweg
Publication year - 2005
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1097/01.mib.0000175906.77340.15
Subject(s) - lactobacillus rhamnosus , lactobacillus reuteri , colitis , antibiotics , cytokine , lactobacillus , medicine , histopathology , mesenteric lymph nodes , cecum , immunology , microbiology and biotechnology , gastroenterology , biology , immune system , pathology , bacteria , genetics
Abstract Background: Scid mice transplanted with CD4 + T blast cells develop colitis. We investigated if the disease was influenced in colitic mice treated with antibiotic and fed Lactobacillus spp . Methods: Colitic scid mice were treated for 1 week with antibiotics (vancomycin/meropenem) followed or not followed by a 3‐week administration of Lactobacillus reuteri DSM‐12246 and Lactobacillus rhamnosus 19070‐2 at 2 × 10 9 live bacteria/mouse/24 hours. After 12 weeks, the rectums were removed for histology, and CD4 + T cells from the mesenteric lymph nodes (MLN) were polyclonally activated for cytokine measurements. Results: Irrespective of no treatment or treatments with antibiotics and probiotics, all mice transplanted with T cell blasts lost 10% of their body weight during the 12‐week experimental period, whereas the nontransplanted mice had a 10% weight increase ( P < 0.001). All mice treated with antibiotics but not fed probiotics showed severe gut inflammation, whereas only 2 of the 7 mice fed probiotics showed signs of severe colitis ( P < 0.05). MLN‐derived CD4 + T cells from this latter group of mice showed lower levels of interleukin‐4 secretion ( P < 0.05) and a tendency to higher interferon‐γ production than mice not fed probiotics. Conclusions: Our data suggest that probiotics added to the drinking water may ameliorate local histopathological changes and influence local cytokine levels in colitic mice but not alter the colitis‐associated weight loss.