Open AccessComputerized Image Analysis of Sirius Red–Stained Renal Allograft Biopsies as a Surrogate Marker to Predict Long-Term Allograft Function
Author(s) -
Paul Grimm,
Peter Nickerson,
James Gough,
Rachel M. McKenna,
Elzbieta Stern,
John Jeffery,
David Rush
Publication year - 2003
Publication title -
journal of the american society of nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.451
H-Index - 279
eISSN - 1533-3450
pISSN - 1046-6673
DOI - 10.1097/01.asn.0000066143.02832.5e
Subject(s) - sirius red , chronic allograft nephropathy , medicine , surrogate endpoint , transplantation , urology , pathology , biopsy , fibrosis , kidney disease , renal function , kidney transplantation , nephropathy , gastroenterology , endocrinology , diabetes mellitus
. Chronic allograft nephropathy (CAN) is a major problem in posttransplant management. The lack of a reliable and early surrogate marker of CAN has hampered patient care and research. In this study, the Cortical Fractional Interstitial Fibrosis Volume (V IntFib ), quantitated with computerized image analysis of Sirius Red–stained protocol biopsies, was examined as a potential surrogate for time to graft failure (TTGF) in 68 renal allograft recipients. At 6 mo posttransplant, V IntFib was highly correlated with TTGF ( r = 0.64, P < 0.001). Both the Banff Chronic Sum and the Acute Sum Scores were also correlated with TTGF, but less strongly ( r = 0.28, P < 0.02; r = 0.35, P < 0.003, respectively). As V IntFib was not correlated with the Banff Chronic Score, a multivariate model was created that incorporated V IntFib and both Acute and Chronic Banff pathology. This model was highly correlated with TTGF ( r = 0.7, P < 0.0001). These findings suggest that V IntFib determined by computerized image analysis of Sirius Red–stained protocol biopsies at 6 mo posttransplant, with or without incorporation of Banff acute and chronic scoring, may provide an early surrogate for time to graft failure in renal allograft recipients. E-mail: pgrimm@ucsd.edu