Open AccessExpression of inducible and endothelial nitric oxide synthases in pouchitis
Author(s) -
Vento Pälvi,
Kiviluoto Tuula,
Järvinen Heikki J.,
Kärkkäinen Päivi,
Kivilaakso Eero,
Soinila Seppo
Publication year - 2001
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1097/00054725-200105000-00007
Subject(s) - pouchitis , gastroenterology , nitric oxide synthase , medicine , ulcerative colitis , inflammation , asymptomatic , pathology , nitric oxide , disease
To study the induction of nitric oxide synthase (NOS) in different forms of pouchitis, we divided patients in five groups: 1) ulcerative colitis, no pouch; 2) no‐pouchitis; 3) chronic asymptomatic pouchitis; 4) chronic active pouchitis; and 5) acute pouchitis. Ileal biopsies were scored for NOS‐2 (inducible) and NOS‐3 (endothelial) immunoreactivity and acute inflammation. In group 1, most specimens lacked NOS‐2 immunoreactivity. In group 2, some specimens showed NOS‐2 immunoreactive epithelium. In group 3, areas of NOS‐2‐immunoreactive epithelium were consistently observed in most specimens. In groups 4 and 5, most specimens showed moderate‐to‐extensive epithelial NOS‐2 staining. NOS‐2 immunoreactivity scores of groups 1–5 were 0.25 ± 0.16, 0.67 ± 0.19, 1.19 ± 0.40, 2.0 ± 0.23, and 2.18 ± 0.12, respectively. Corresponding acute inflammation scores were 0, 0.53 + 0.17, 1.00 ± 0.33, 1.80 ± 0.20, and 1.64 ± 0.15. NOS‐2 score correlated with acute inflammation score (p < 0.0001), indicating that NOS‐2 induction correlates with both the clinical degree of pouchitis and the severity of acute inflammation. NOS‐3 immunoreactivity increased in all pouchitis groups.