Dominant Bovine Ovarian Follicular Cysts Express Increased Levels of Messenger RNAs for Luteinizing Hormone Receptor and 3β-Hydroxysteroid Dehydrogenase Δ4,Δ5 Isomerase Compared to Normal Dominant Follicles | Zendy

Michele D. Calder | Zendy; Mohan Manikkam | Zendy; B. E. Salfen | Zendy; R.S. Youngquist | Zendy; Dennis B. Lubahn | Zendy; W. R. Lamberson | Zendy; H.A. Garverick | Zendy

Open Access

Dominant Bovine Ovarian Follicular Cysts Express Increased Levels of Messenger RNAs for Luteinizing Hormone Receptor and 3β-Hydroxysteroid Dehydrogenase Δ4,Δ5 Isomerase Compared to Normal Dominant Follicles

Author(s) -

Michele D. Calder,

Mohan Manikkam,

B. E. Salfen,

R.S. Youngquist,

Dennis B. Lubahn,

W. R. Lamberson,

H.A. Garverick

Publication year - 2001

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod65.2.471

Subject(s) - biology , medicine , endocrinology , follicular phase , androstenedione , aromatase , cholesterol side chain cleavage enzyme , hydroxysteroid dehydrogenase , follicular fluid , ovarian follicle , progesterone receptor , luteinizing hormone , estrogen , estrogen receptor , gonadotropin , receptor , hormone , cytochrome p450 , dehydrogenase , androgen , oocyte , enzyme , metabolism , microbiology and biotechnology , embryo , biochemistry , genetics , cancer , breast cancer

The objective was to compare ovarian steroids and expression of mRNAs encoding cytochrome P450 side-chain cleavage, cytochrome P450 17 alpha-hydroxylase, cytochrome P450 aromatase, 3 beta-hydroxysteroid dehydrogenase Delta(4),Delta(5) isomerase, LH, and FSH receptors and estrogen receptor-beta in ovaries of cows with dominant and nondominant ovarian follicular cysts and in normal dominant follicles. Estradiol-17 beta, progesterone, and androstenedione concentrations were determined in follicular fluid using specific RIAs. Dominant cysts were larger than young cysts or dominant follicles, whereas nondominant cysts were intermediate. Estradiol-17 beta (ng/ml) and total steroids (ng/follicle) were higher in dominant cysts than in dominant follicles. Expression of LH receptor and 3 beta-hydroxysteroid dehydrogenase mRNAs was higher in granulosa cells of dominant cysts than in dominant follicles. Nondominant cysts had higher follicular concentrations of progesterone, lower estradiol-17 beta concentrations, and lower expression of steroidogenic enzyme, gonadotropin receptor, and estrogen receptor-beta mRNAs than other groups. In summary, increased expression of LH receptor and 3 beta-hydroxysteroid dehydrogenase mRNAs in granulosa and increased follicular estradiol-17 beta concentrations were associated with dominant cysts compared to dominant follicles. Study of cysts at known developmental stages is useful in identifying alterations in follicular steroidogenesis.

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