Open AccessExpression of Selenoprotein-P Messenger Ribonucleic Acid in the Rat Testis
Author(s) -
Minoru Koga,
Hiromitsu Tanaka,
Kentaro Yomogida,
Junji Tsuchida,
Kinya Uchida,
Masaya Kitamura,
Saburo Sakoda,
Kiyomi Matsumiya,
Äkïhïko Okuyama,
Yoshitake Nishimune
Publication year - 1998
Publication title -
biology of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.366
H-Index - 180
eISSN - 1529-7268
pISSN - 0006-3363
DOI - 10.1095/biolreprod58.1.261
Subject(s) - selenoprotein , biology , northern blot , messenger rna , selenoprotein p , testicle , in situ hybridization , western blot , microbiology and biotechnology , leydig cell , medicine , endocrinology , biochemistry , gene , catalase , oxidative stress , glutathione peroxidase , luteinizing hormone , hormone
It has been suggested that a plasma protein, selenoprotein P, functions as an antioxidant and that its mRNA is expressed ubiquitously, including in the testis. To determine its physiological function, we have investigated the expression of selenoprotein-P mRNA in the rat testis. Northern blot analysis showed that selenoprotein P was exclusively expressed in the Leydig cell fraction. In situ hybridization experiments further supported this observation. Testes of rats administered ethylene dimethane sulfonate (EDS) were also examined by Northern blot analysis. Selective degeneration of Leydig cells by EDS treatment resulted in disappearance of selenoprotein-P mRNA from the testis. Furthermore, upon recovery, in association with regenerative differentiation of Leydig cells, reappearance of the selenoprotein-P mRNA was observed. These results indicated that selenoprotein-P mRNA was predominantly expressed in the interstitial Leydig cells.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom