English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Adult rat brains contain LH/hCG receptors, and these receptors are functional in neuroendocrine regulation and behaviors. Since glial cells are important for development, maturation, and functioning of the brain, we tested the hypothesis that these cells from neonatal rat brains may also contain functional LH/hCG receptors. Reverse-transcriptase polymerase chain reaction amplified an expected 256 base-pair LH/hCG receptor fragment from glial cells. This fragment can bind to LH/hCG receptor cDNA in Southern blotting. Northern blotting demonstrated that glial cells contain a major 2.6-kilobase (kb) and a minor 4.3-kb transcript of LH/hCG receptors. Western immunoblotting demonstrated that glial cells also contain an 80-kDa receptor protein and that its levels are significantly higher in secondary and tertiary glial cells than in primary glial cells. Immunocytochemistry confirmed that LH/hCG receptor immunostaining is present in glial cells. Since glial cells are quite active in synthesis of prostaglandins (PGs), we investigated the effect of highly purified hCG on PGD2 and PGE2 levels. The results showed that culturing secondary glial cells for three days with highly purified hCG resulted in a dose-dependent and hormone-specific increase in PGD2 and a decrease in PGE2 levels in the medium as compared to control levels. In summary, we conclude that cultured glial cells from neonatal rat brains contain functional LH/hCG receptors. Through regulation of PG synthesis, LH and hCG may influence glial cell functions that are important for neonatal brain development and function.

Novel Expression of Functional Luteinizing Hormone/Chorionic Gonadotropin Receptors in Cultured Glial Cells From Neonatal Rat Brains1