Localization of Preovulatory Expression of Plasminogen Activator Inhibitor Type-1 and Tissue Inhibitor of Metalloproteinase Type-1 mRNAs in the Rat Ovary1 | Zendy

S-Y. Chun | Zendy; Malka Popliker | Zendy; Reuven Reich | Zendy; A. Tsafriri | Zendy

Open Access

Localization of Preovulatory Expression of Plasminogen Activator Inhibitor Type-1 and Tissue Inhibitor of Metalloproteinase Type-1 mRNAs in the Rat Ovary1

Author(s) -

S-Y. Chun,

Malka Popliker,

Reuven Reich,

A. Tsafriri

Publication year - 1992

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod47.2.245

Subject(s) - theca , biology , follicular phase , ovarian follicle , endocrinology , medicine , ovulation , theca interna , plasminogen activator , in situ hybridization , folliculogenesis , tissue inhibitor of metalloproteinase , ovary , granulosa cell , follicle , plasminogen activator inhibitor 1 , messenger rna , microbiology and biotechnology , extracellular matrix , embryo , embryogenesis , hormone , biochemistry , gene

Proteinases and their inhibitors control follicular connective tissue remodeling associated with follicular rupture. We examined the regulation and cellular localization of plasminogen activator inhibitor type-1 (PAI-1) and tissue inhibitor of metalloproteinase type-1 (TIMP-1) mRNAs by in situ hybridization. [35S]UTP-labeled RNA probes were hybridized to ovarian sections of eCG-primed immature rats treated with hCG. Before hCG stimulation of ovulation, very low expression of PAI-1 mRNA was observed in theca cells. After hCG administration, expression of PAI-1 mRNA was increased in theca cells of most antral follicles, whereas expression in granulosa cells was limited to preovulatory follicles and only to areas where the basal membrane was dissociated. Before hCG treatment, low expression of TIMP-1 mRNA was observed in theca cells, but not in granulosa cells. After hCG treatment, TIMP-1 mRNA was greatly stimulated in theca cells irrespective of follicle size, while the expression in granulosa cells was limited to large antral follicles. The present study demonstrates cell-specific expression of PAI-1 and TIMP-1 mRNAs in the LH/hCG-stimulated ovary, thus confirming the localized control of preovulatory proteolysis by coexpression of both enzymes and their respective inhibitors.

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