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This study was undertaken to determine the effect of the implanting cat blastocyst on endometrial morphology and protein synthesis. Placental and endometrial tissues were obtained from pregnant and pseudopregnant cats and then cultured with L-[35S]methionine and analyzed for protein synthesis by 2-dimensional polyacrylamide gel electrophoresis followed by fluorography, and also processed for light microscopy. The progesterone-dependent protein (PDP), described previously by Boomsma and Verhage (Biol Reprod 1987; 37:117-126) and Verhage et al. (Biol Reprod 1989; 41:347-354), was identified by immunocytochemical and immunoblot analysis. Attachment began after 12 days, and the deep glands contained large deposits of PDP. By 20 days the placenta was well developed, and the deep endometrial glands under the placenta had regressed and lacked deposits of PDP. The placenta continued to develop and thicken as pregnancy progressed. The surface epithelium in the non-implantation site regions developed extreme convolutions, while the well-developed deep glands with large deposits of PDP began to regress by 4 weeks, becoming similar to those in the implantation site. The endometrial glands in the pseudopregnant animals maintained deposits of PDP even though apoptotic bodies were observed between 20 and 35 days. PDP synthesis was not detected in the implantation site after 16 days, but it continued in the nonimplantation site through 5 weeks. The synthesis of nine other proteins was significantly altered by the end of implantation such that the pattern in the non-site endometrium was different from the implantation site but similar to the pattern found in the pseudopregnant endometrium. As pregnancy progressed, protein synthesis was altered in the placental/junctional zone and the non-site endometrium, but in the deep endometrial portion of the implantation site it was largely unchanged and similar to the deep portion of the non-site. Thus, the implanting cat blastocyst has a significant effect on the morphology of the implantation site and non-site endometrium, and alters the protein synthetic activity of the implantation site endometrium but apparently not the non-site region. The morphology and protein synthetic patterns of the pregnant cat uterus show regional differentiation and continue to change as pregnancy progresses.

Changes in Endometrial and Placental Protein Synthesis and Morphology during Pregnancy and Pseudopregnancy in the Cat1