Methods for Evaluating the Acrosomal Status of Mammalian Sperm1 | Zendy

Nicholas L. Cross | Zendy; Stanley Meizel | Zendy

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Methods for Evaluating the Acrosomal Status of Mammalian Sperm1

Author(s) -

Nicholas L. Cross,

Stanley Meizel

Publication year - 1989

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod41.4.635

Subject(s) - biology , acrosome reaction , sperm , acrosome , fluorescence microscope , microscopy , microbiology and biotechnology , fluorescence , pathology , optics , genetics , medicine , physics

A full understanding of the acrosome reaction is central to understanding sperm function. Acrosomal status can be determined on living, motile sperm in only a few mammalian species. For other species, many light microscopic methods have been developed, including colored stains for bright-field microscopy, and probes for fluorescence microscopy. We review the existing methods and the criteria that should be considered in the choice of an assay.

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