Sex Preselection in Rabbits: Live Births from X and Y Sperm Separated by DNA and Cell Sorting | Zendy

Lawrence A. Johnson | Zendy; J. P. Flook | Zendy; H. W. Hawk | Zendy

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Sex Preselection in Rabbits: Live Births from X and Y Sperm Separated by DNA and Cell Sorting

Author(s) -

Lawrence A. Johnson,

J. P. Flook,

H. W. Hawk

Publication year - 1989

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod41.2.199

Subject(s) - sperm , biology , andrology , insemination , offspring , semen , population , artificial insemination , y chromosome , genetics , pregnancy , gene , medicine , demography , sociology

Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.

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