Identification of the Specific Proteins Associated with Differentiation of Spermatogonia in Mice by Two-Dimensional Gel Electrophoresis | Zendy

Kazuhiro Sakamaki | Zendy; Ken Sawada | Zendy; Yoshitake Nishimune | Zendy

Open Access

Identification of the Specific Proteins Associated with Differentiation of Spermatogonia in Mice by Two-Dimensional Gel Electrophoresis

Author(s) -

Kazuhiro Sakamaki,

Ken Sawada,

Yoshitake Nishimune

Publication year - 1987

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod37.4.989

Subject(s) - biology , isoelectric point , isoelectric focusing , gel electrophoresis , microbiology and biotechnology , two dimensional gel electrophoresis , electrophoresis , molecular mass , pi , proteomics , biochemistry , gene , enzyme

Protein synthesis in testicular germ cells was studied during the differentiation of Type A spermatogonia in testes from adult cryptorchid mice to Intermediate and Type B spermatogonia in organ culture by two-dimensional gel electrophoresis. By comparing the patterns of Type A spermatogonia with those of differentiated (Intermediate and Type B) spermatogonia, we found two newly synthesized protein spots that correlated with differentiation. The isoelectric points (pIs) and molecular weights (MWs) of these proteins were estimated to be approximately pI 5.4, MW 67,000, and pI 5.8, MW 28,000, respectively.

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