Prevention of Spontaneous Degeneration of Mouse Oocytes in Culture by Ovarian Glycosaminoglycans1 | Zendy

Eimei Sato | Zendy; Takehiko ISHIBASHI | Zendy; S. S. Koide | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Prevention of Spontaneous Degeneration of Mouse Oocytes in Culture by Ovarian Glycosaminoglycans1

Author(s) -

Eimei Sato,

Takehiko ISHIBASHI,

S. S. Koide

Publication year - 1987

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod37.2.371

Subject(s) - chondroitin sulfate , hyaluronic acid , follicular fluid , glycosaminoglycan , in vitro , biology , follicular phase , andrology , embryo , oocyte , microbiology and biotechnology , biochemistry , anatomy , endocrinology , medicine

Mouse follicular oocytes cultured for more than 72 h in modified Krebs-Ringer solution in vitro undergo spontaneous segmentation to form "blastomeres" with or without nuclear fragments or chromatin. Glycosaminoglycans (GAGS) isolated from bovine follicular fluid (bFF) and added to the suspending medium at concentrations of 0.25 mg/ml or greater prevented the occurrence of spontaneous segmentation in isolated cumulus-free mouse oocytes in a dose-dependent manner. The active material was isolated from bFF by chromatography on Dowex 1-X2. The fraction eluted with 0.5 M NaCl possessed the greatest biological activity. Preparations of purified bFF-GAGs and hyaluronic acid and chondroitin sulfate from a commercial source prevented the occurrence of segmentation. Hyaluronic acid was more potent than chondroitin sulfate. The present results show that GAGs prevent segmentation of oocytes in vitro.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research