Dehydroepiandrosterone Sulfate Stimulates Cal lagenase Synthesis without Affecting the Rates of Collagen and Noncollagen Protein Syntheses by Rabbit Uterine Cervical Fibroblasts | Zendy

Katsufumi Sakyo | Zendy; Akira Itô | Zendy; Yo Mori | Zendy

Open Access

Dehydroepiandrosterone Sulfate Stimulates Cal lagenase Synthesis without Affecting the Rates of Collagen and Noncollagen Protein Syntheses by Rabbit Uterine Cervical Fibroblasts

Author(s) -

Katsufumi Sakyo,

Akira Itô,

Yo Mori

Publication year - 1987

Publication title -

biology of reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.366

H-Index - 180

eISSN - 1529-7268

pISSN - 0006-3363

DOI - 10.1095/biolreprod36.2.277

Subject(s) - biology , collagenase , endocrinology , dna synthesis , medicine , protein biosynthesis , stimulation , catabolism , thymidine , dehydroepiandrosterone , biochemistry , microbiology and biotechnology , in vitro , enzyme , metabolism , hormone , androgen

The effects of dehydroepiandrosterone 3-sulfate (DHAS) and 17 beta-estradiol (E2) on collagen and noncollagen protein syntheses by rabbit uterine cervical cells were studied, and their effects on latent collagenase synthesis were compared. DHAS (1 X 10(-6) M) stimulated the synthesis of latent collagenase and did not affect the cell number and [3H]thymidine incorporation into DNA, whereas E2 had no effect on collagenase synthesis. On the other hand, neither DHAS (1 X 10(-6) M) nor E2 (1 X 10(-10)-1 X 10(-6) M) showed effects on collagen and noncollagen protein syntheses. These results suggest that the stimulative effect of DHAS on cervical ripening is mediated mainly by the stimulation of collagen catabolism, and that E2 does not concern the changes in the concentration of collagen and noncollagen protein in uterine cervix of the rabbit during pregnancy at term.

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