Compensatory Ovarian Hypertrophy in the Long-Term Hemicastrate Rat: Size Distribution of Growing and Atretic Follicles

In the intact rat, on estrus, the follicle-stimulating hormone (FSH) surge recruits nearly twice the correct number of follicles for ovulation, then, on metestrus, the excess follicles undergo atresia. In contrast, in the long-term hemicastrate rat, the FSH surge recruits fewer antral follicles on estrus, but there is little atresia on metestrus. To determine if fewer follicles are recruited by the FSH surge of long-term hemicastrates because the pool of follicles capable of responding to the FSH is smaller than in intact rats, preantral, antral, atretic, and healthy follicles were counted in ovaries of rats killed on each day of the estrous cycle. In general, there were only half as many healthy preantral follicles per rat in hemicastrates compared with intacts. There were an equal number of large antral follicles per rat in hemicastrates compared with intacts. Thus, compensatory hypertrophy did not extend to preantral follicles but was evident in large preovulatory follicles. These results suggest that fewer follicles are recruited on estrus in hemicastrate rats because fewer follicles are at the appropriate stage of development to respond to the FSH surge.