A Reovirus Causes Hypovirulence of Rosellinia necatrix | Zendy

Satoko Kanematsu | Zendy; Masaaki Arakawa | Zendy; Yuri Oikawa | Zendy; Mari Onoue | Zendy; H. Osaki | Zendy; Hitoshi Nakamura | Zendy; Kenichi Ikeda | Zendy; Y. Kuga-Uetake | Zendy; Hiromichi Nitta | Zendy; Akihiro Sasaki | Zendy; Koichi Suzaki | Zendy; Kouji Yoshida | Zendy; Naoyuki Matsumoto | Zendy

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A Reovirus Causes Hypovirulence of Rosellinia necatrix

Author(s) -

Satoko Kanematsu,

Masaaki Arakawa,

Yuri Oikawa,

Mari Onoue,

H. Osaki,

Hitoshi Nakamura,

Kenichi Ikeda,

Y. Kuga-Uetake,

Hiromichi Nitta,

Akihiro Sasaki,

Koichi Suzaki,

Kouji Yoshida,

Naoyuki Matsumoto

Publication year - 2004

Publication title -

phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.264

H-Index - 131

eISSN - 1943-7684

pISSN - 0031-949X

DOI - 10.1094/phyto.2004.94.6.561

Subject(s) - biology , inoculation , virulence , rna silencing , strain (injury) , mycovirus , microbiology and biotechnology , hypha , subculture (biology) , virology , botany , rna , horticulture , genetics , gene , anatomy , rna interference , rna polymerase

White root rot, caused by Rosellinia necatrix, is a serious soilborne disease of fruit trees and other woody plants. R. necatrix isolate W370 contains 12 segments of double-stranded RNA (dsRNA) that is believed to represent a possible member of the family Reoviridae. W370 was weakly virulent and its hyphal-tip strains became dsRNA free and strongly virulent. The 12 segments of W370dsRNA were transmitted to hygromycin B-resistant strain RT37-1, derived from a dsRNA-free strain of W370 in all or none fashion through hyphal contact with W370. The W370dsRNA-transmitted strains were less virulent than their parent strain RT37-1 on apple seedlings, with mortality ranging between 0 to 16.7% in apple seedlings that were inoculated with the W370dsRNA-containing strains and 50 to 100% for seedlings inoculated with the dsRNA-free strains. Some W370dsRNA-containing strains killed greater than 16.7% of seedlings, but these were found to have lost the dsRNA in planta. These results indicate that W370dsRNA is a hypovirulence factor in R. necatrix. In addition, a strain lost one segment (S8) of W370dsRNA during subculture, and the S8-deficient mutant strain also exhibits hypovirulence in R. necatrix.

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