Open AccessInvolvement of Phosphoglucose Isomerase in Pathogenicity of Xanthomonas oryzae pv. oryzae
Author(s) -
Seiji Tsuge,
Hirokazu Ochiai,
Yasuhiro Inoue,
Takashi Oku,
Kazunori Tsuno,
Hisatoshi Kaku,
Yasuyuki Kubo
Publication year - 2004
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.2004.94.5.478
Subject(s) - xanthomonas oryzae pv. oryzae , xanthomonas oryzae , biology , mutant , transposable element , gene , virulence , transposon mutagenesis , rhizopus oryzae , blight , microbiology and biotechnology , mutagenesis , cloning (programming) , genetics , botany , biochemistry , fermentation , computer science , programming language
Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, was subjected to transposon mutagenesis to generate mutants defective in pathogenicity. A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf blight-resistant rice and tomato. Cloning and sequence analysis revealed that the transposon in 74M913 was inserted in a gene homologous to the phosphoglucose isomerase (pgi) gene of X. axonopodis pv. citri. Growth of the mutant in a synthetic medium containing fructose or xylose as a sole carbohydrate source was much reduced, indicating the transposon disrupted pgi function. The interaction between expression of pgi and hypersensitive response and pathogenicity (hrp) genes was investigated because we had demonstrated previously that expression of hrp genes of X. oryzae pv. oryzae is induced in a synthetic medium containing xylose. However, pgi and the hrp gene (hrcU) were expressed independently. This study suggests that PGI is involved in pathogenicity of X. oryzae pv. oryzae.