Open AccessInduction of Chitinase, β-1,3-Glucanase, and Phenylalanine Ammonia Lyase in Peach Fruit by UV-C Treatment
Author(s) -
Ahmed El Ghaouth,
Charles L. Wilson,
Ann M. Callahan
Publication year - 2003
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.2003.93.3.349
Subject(s) - chitinase , phenylalanine ammonia lyase , glucanase , elicitor , biology , phenylalanine , lyase , enzyme , horticulture , botany , biochemistry , microbiology and biotechnology , amino acid
Treatment of peach fruit with UV-C light caused a rapid induction of chitinase, β-1,3-glucanase, and phenylalanine ammonia lyase (PAL) activities starting 6 h after treatment and reaching maximum levels at 96 h after treatment. By 96 h after UV-C treatment, chitinase, β-1,3-glucanase, and PAL activities in UV-C-treated fruit were over twofold above the levels observed for the control. In nontreated control fruit, no apparent increase in chitinase and β-1,3-glucanase activities was detected but a minor increase in PAL activity was seen. The transient increase in chitinase, β-1,3-glucanase, and PAL activities in UV-C-treated fruit was preceded by a gradual activation of the corresponding genes. UV-C-treated fruit showed an increase in accumulation of β-1,3-glucanase and chitinase mRNAs at 3 h after treatment, which peaked approximately 96 h posttreatment. A similar induction kinetic pattern was observed for PAL mRNA in response to UV-C treatment, except the induction started 6 h after UV-C treatment. These results show that the response of peach fruit to elicitor treatment is similar to that seen in other plant-elicitors interactions and suggests the involvement of peach biochemical defense responses in UV-C-mediated disease resistance.