Open AccessIdentification of Plum pox virus Determinants Implicated in Specific Interactions with Different Prunus spp.
Author(s) -
Sylvie Dallot,
L. Quiot-Douine,
Pilar Sáenz,
Marı́a Teresa Cervera,
Juan-Antonio García,
J. B. Quiot
Publication year - 2001
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.2001.91.2.159
Subject(s) - biology , prunus , genome , pox virus , virology , potyviridae , potyvirus , virus , genome size , genetics , plant virus , gene , botany
The characterization of pathogenic properties of two infectious clones of Plum pox virus (PPV) isolates, pGPPV (D group) and pGPPVPS (M group), was investigated in their woody hosts (seedlings of Prunus spp.). The two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. The phenotype determinants were located with a set of chimeric viruses from the two clones. In plum, determinants of systemic infection were located in a genomic fragment encoding the P3 and 6K1 proteins, which might influence genome amplification or virus movement. The capacity of pGPPVPS to induce stable local and systemic infections in peach was not located accurately and might be influenced by multiple determinants carried by different regions of the genome, excluding those encoding the protein 1, the majority of helper component, nuclear inclusions a and b, and coat protein. We conclude that PPV infections of plum and peach are governed by different determinants.