Bulked Segregant Analysis Identifies Molecular Markers Linked to Melampsora medusae Resistance in Populus deltoides | Zendy

Girma Tabor | Zendy; Thomas L. Kubisiak | Zendy; Ned B. Klopfenstein | Zendy; Richard B. Hall | Zendy; H. S. McNabb McNabb | Zendy

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Bulked Segregant Analysis Identifies Molecular Markers Linked to Melampsora medusae Resistance in Populus deltoides

Author(s) -

Girma Tabor,

Thomas L. Kubisiak,

Ned B. Klopfenstein,

Richard B. Hall,

H. S. McNabb McNabb

Publication year - 2000

Publication title -

phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.264

H-Index - 131

eISSN - 1943-7684

pISSN - 0031-949X

DOI - 10.1094/phyto.2000.90.9.1039

Subject(s) - biology , bulked segregant analysis , centimorgan , locus (genetics) , rapd , genetics , genetic marker , molecular marker , gene mapping , botany , gene , chromosome , genetic diversity , population , demography , sociology

ABSTRACT In the north central United States, leaf rust caused by Melampsora medusae is a major disease problem on Populus deltoides. In this study we identified molecular markers linked to a M. medusae resistance locus (Lrd1) that was segregating 1:1 within an intraspecific P. deltoides family (C9425DD). Previous field results were confirmed in the controlled environment of a growth chamber through an excised whole-leaf inoculation method. Using bulked segregant analysis we identified two random amplified polymorphic DNA (RAPD) markers (OPG10(340) and OPZ19(1800)) that are linked to Lrd1. Based on segregation in a total of 116 progeny, the genetic distances between OPG10(340) and OPZ19(1800) and the resistance locus were estimated as 2.6 and 7.4 Haldane centimorgans (cM), respectively. Multipoint linkage analyses strongly suggest the most likely order for these loci is Lrd1, OPG10(340), and OPZ19(1800). These markers may prove to be instrumental in the eventual cloning of Lrd1, as well as for marker-assisted selection of leaf-rust resistant genotypes.

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