Open AccessRapid Detection of the Fusarium oxysporum Lineage Containing the Canary Island Date Palm Wilt Pathogen
Author(s) -
T.R. Plyler,
G. W. Simone,
Diana Fernandez,
H. Corby Kistler
Publication year - 1999
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.1999.89.5.407
Subject(s) - biology , fusarium oxysporum , genomic dna , polymerase chain reaction , pathogen , fusarium wilt , lineage (genetic) , botany , microbiology and biotechnology , dna , gene , genetics
Fusarium oxysporum f. sp. canariensis causes Fusarium wilt disease on the Canary Island date palm (Phoenix canariensis). To facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. A partial genomic library of F. oxysporum f. sp. canariensis isolate 95-913 was used to identify a DNA sequence diagnostic for a lineage containing all tested isolates of F. oxysporum f. sp. canariensis. Two oligonucleotide primers were designed and used to amplify a 567-bp fragment with F. oxysporum f. sp. canariensis DNAs. DNA from 61 outgroup isolates did not amplify using these primers. Once the primers were shown to amplify a 0.567-kb fragment from DNA of all the F. oxysporum f. sp. canariensis isolates tested, a rapid DNA extraction procedure was developed that led to the correct identification of 98% of the tested F. oxysporum f. sp. canariensis isolates.