Open AccessIdentification, Characterization, and Relatedness of Luteovirus Isolates from Forage Legumes
Author(s) -
V. D. Damsteegt,
Andrew L. Stone,
Anthony J. Russo,
Douglas G. Luster,
F. E. Gildow,
O. P. Smith
Publication year - 1999
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.1999.89.5.374
Subject(s) - biology , luteovirus , acyrthosiphon pisum , polyclonal antibodies , antiserum , virology , fabaceae , dwarfing , plant virus , botany , aphididae , virus , genetics , gene , antibody , homoptera , pest analysis
Virus isolates from forage legumes collected from eight different states were identified as luteoviruses closely related to soybean dwarf luteovirus dwarfing (SbDV-D) and yellowing (SbDV-Y) described in Japan. All isolates produced reddened leaf margins in subterranean clover and were transmitted in a persistent manner by Acrythosiphon pisum, but not by Aulacorthum solani. Specific monoclonal antibodies raised against SbDV-Y were differentially reactive with endemic isolates. Immunoblots probed with a SbDV-D polyclonal antiserum showed single 26-kDa coat protein bands, confirming close serological relatedness to SbDV. Analyses of genomic and subgenomic double-stranded RNAs and northern blot analyses confirmed genomic relatedness to SbDV. Based on our results, we conclude that the U.S. luteovirus isolates studied comprise a strain or strains of the soybean dwarf virus that have clovers as common hosts and the pea aphid as a common vector.