Open AccessPhytoalexin Production in an Apple Cultivar Resistant to Venturia inaequalis
Author(s) -
G. Hrazdina,
Wlodzimierz Borejsza-Wysocki,
Cathy Lester
Publication year - 1997
Publication title -
phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.264
H-Index - 131
eISSN - 1943-7684
pISSN - 0031-949X
DOI - 10.1094/phyto.1997.87.8.868
Subject(s) - venturia inaequalis , phytoalexin , biology , apple scab , malus , mycelium , cultivar , spore germination , horticulture , botany , spore , biochemistry , fungicide , resveratrol
Cell suspension cultures of the scab-resistant apple (Malus × domestica) cultivar Liberty were challenged with yeast extract to mimic the effect of biological stress such as fungal invasion. The cells responded to the challenge by production of novel compounds. Suspension cultures of the scab-susceptible cultivar McIntosh, when similarly challenged, showed no detectable response. The major compound produced by scab-resistant cells in response to the challenge has been identified as the 2,4-methoxy-3-hydroxy-9-O-β-D-glucosyloxydibenzofuran by UV, mass spectrometry, 1 H-nuclear magnetic resonance (NMR), and 13 C-NMR spectroscopy. We suggest the trivial name malusfuran for the compound. Malusfuran production was initiated approximately 24 h after being challenged. Malusfuran inhibited spore germination and growth of Venturia inaequalis at millimolar concentrations, indicating its role as a possible phytoalexin. The aglycone of malusfuran, 2,4-methoxy-3,9-hydroxy-dibenzofuran, showed higher toxicity to V. inaequalis than to the parent malusfuran. In vitro cultures of V. inaequalis produced a β-glucosidase that hydrolyzed ortho- and para-substituted nitrophenyl-β-glucosides, suggesting that the aglycone may act as the actual phytoalexin.