Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice | Zendy

Chaoxi Luo | Zendy; LiangFen Yin | Zendy; Satoru Koyanagi | Zendy; Mark Farman | Zendy; Motoaki Kusaba | Zendy; Hiroshi Yaegashi | Zendy

Open Access

Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice

Author(s) -

Chaoxi Luo,

LiangFen Yin,

Satoru Koyanagi,

Mark Farman,

Motoaki Kusaba,

Hiroshi Yaegashi

Publication year - 2005

Publication title -

phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.264

H-Index - 131

eISSN - 1943-7684

pISSN - 0031-949X

DOI - 10.1094/phyto-95-0640

Subject(s) - biology , genetics , chromosome , rapd , genetic linkage , gene mapping , gene , genetic marker , karyotype , chromosome 4 , autosome , microbiology and biotechnology , genetic diversity , population , demography , sociology

A genetic map including three avirulence (Avr) genes, AvrPik, AvrPiz, and AvrPiz-t, was constructed in a genetic cross of two rice field isolates, 84R-62B and Y93-245c-2. The chromosomal locations of the Avr genes were determined by using selected markers to probe Southern blots of the parental chromosomes that had been separated by contour-clamped homogenous electric fields electrophoresis. Electrophoretic karyotyping showed that both parental isolates 84R-62B and Y93-245c-2 contained seven chromosomes greater than 3.5 megabases (Mb) in size and 84R-62B possessed a small chromosome of ≈1.6 Mb. The linkage groups containing AvrPiz and AvrPiz-t were assigned to chromosomes 3 and 7, respectively. Some markers from the linkage group that contained AvrPik hybridized with chromosome 1 and the 1.6-Mb chromosome, yet all of the cloned RAPD markers that were closely linked to AvrPik hybridized exclusively to the 1.6-Mb chromosome in 84R-62B, the parent that possesses AvrPik. Thus, we conclude that AvrPik is located on the 1.6-Mb chromosome in 84R-62B.

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