Open AccessPostharvest Biological Control of Rhizopus Rot of Nectarine Fruits by Pichia membranefaciens
Author(s) -
QingHua Fan,
Shiping Tian
Publication year - 2000
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis.2000.84.11.1212
Subject(s) - rhizopus , iprodione , biology , yeast , inoculation , postharvest , botrytis cinerea , mycelium , horticulture , biological pest control , spore , microbiology and biotechnology , fungicide , botany , food science , fermentation , biochemistry
A new yeast antagonist, Pichia membranefaciens, isolated from wounds of peach fruit, was evaluated for its biocontrol capability against Rhizopus stolonifer on nectarine fruits at different temperatures and with other treatments. P. membranefaciens at 5 × 10 8 CFU/ml of washed-cell suspension completely inhibited Rhizopus rot in nectarine wounds artificially inoculated with 5 × 10 4 spores per ml at 25, 15, and 3°C. A culture filtrate of the yeast antagonist failed to provide any protection against Rhizopus rot in nectarine fruits. The yeast mixed with iprodione at 100 μg a.i./ml gave better control of R. stolonifer than either yeast or iprodione alone. A solution of 20 g CaCl 2 per liter enhanced the efficacy of P. membranefaciens (10 7 to 10 8 CFU/ml) as an aqueous suspension. Rapid colonization of the yeast in wounds was observed during the first 48 h at 25 and 15°C. P. membranefaciens at 5 × 10 8 CFU/ml was effective when applied 0 to 72 h before the pathogen, while at 1 × 10 8 CFU/ml, its efficacy was best when applied 24 to 48 h prior to inoculation with R. stolonifer. However, its efficacy was significantly reduced when the yeast was applied simultaneously with the pathogen, with disease incidence of 60% and lesion diameter of 37 mm.