Open AccessComparison of Stenocarpella maydis Isolates for Isozyme and Cultural Characteristics
Author(s) -
Anne E. Dorrance,
Orson K. Miller,
H. L. Warren
Publication year - 1999
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis.1999.83.7.675
Subject(s) - biology , isozyme , malate dehydrogenase , esterase , potato dextrose agar , fungi imperfecti , botany , genetic variability , agar , agar plate , microbiology and biotechnology , horticulture , enzyme , genotype , gene , bacteria , genetics , biochemistry
Isolates of Stenocarpella maydis from seed companies and plant disease clinics in the United States and the Republic of South Africa were assayed for isozyme polymorphisms and cultural variability. A low level of isozyme polymorphisms was detected in this collection of isolates. Isozyme polymorphisms were detected for α-esterase, hexose kinase, and malate dehydroge-nase of the enzymes assayed. Fungi often have limited variability among isozyme profiles, and this is especially true for fungi that have host specialization such as biotrophs or fungi with formae speciales designations. Optimum growth temperature, colony color, and pycnidiospore production were also measured. All isolates had an optimum temperature of 28 to 31°C for colony growth on acidified potato dextrose agar. Colony color and pycnidiospore production were variable over the course of several experiments, indicating that these phenotypes are poor genetic markers.