Open AccessAntiviral and Antiviroid Activity of MAP-Containing Extracts fromMirabilis jalapaRoots
Author(s) -
Jorge M. Vivanco,
Maddalena Querci,
Luis Salazar
Publication year - 1999
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis.1999.83.12.1116
Subject(s) - biology , infectivity , inoculation , virology , virus , plant virus , tobacco mosaic virus , viroid , nucleic acid , botany , antiviral protein , potato virus y , cucumber mosaic virus , potato spindle tuber viroid , rna , horticulture , biochemistry , gene
Extracts of Mirabilis jalapa (Nyctaginaceae), containing a ribosome inactivating protein (RIP) called Mirabilis antiviral protein (MAP), were tested against infection by potato virus X, potato virus Y, potato leaf roll virus, and potato spindle tuber viroid. Root extracts of M. jalapa sprayed on test plants 24 h before virus or viroid inoculation inhibited infection by almost 100%, as corroborated by infectivity assays and the nucleic acid spot hybridization test. Antiviral activity of MAP extracts was observed against mechanically transmitted viruses but not against aphid-transmitted viruses. Purified MAP showed the same antiviral effect as the crude extracts.