Open AccessSeed Transmission ofPepino mosaic virusand Efficacy of Tomato Seed Disinfection Treatments
Author(s) -
M. C. Córdoba-Sellés,
Ana García-Rández,
A. Alfaro-Fernández,
C. Jordá-Gutiérrez
Publication year - 2007
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis-91-10-1250
Subject(s) - biology , lycopersicon , seedling , germination , cotyledon , horticulture , tobamovirus , plant virus , virus , virology
Rates of seed transmission for Pepino mosaic virus (PepMV) were estimated in seedlings grown from seeds obtained from symptomatic tomato (Lycopersicon esculentum) fruits of plants naturally infected with the virus. The proportion of seeds infected with PepMV was at least 25% as estimated from enzyme-linked immunosorbent assay (ELISA) analysis of grouped seeds. The seeds from symptomatic fruits were planted, and seedlings at the cotyledon and transplant stage were assayed for PepMV by ELISA. Three of 168 seedlings grown from infected seeds were PepMV-positive, corresponding to a seed-to-seedling transmission rate of 1.84%. Various tomato seed treatments were evaluated for their ability to prevent seed transmission of PepMV. This virus was largely eradicated by immersing the seeds in 10% trisodium phosphate for 3 h. Although heat treatments of 24 h at 80°C and 48 h at 74°C eliminated PepMV in seedlings, these treatments did not eradicate the virus in whole seeds. The three treatments did not adversely affect seed germination. The results suggest that trisodium phosphate can be used to eradicate PepMV in tomato seed without hindering germination.