Open AccessCadophora sabaouaesp. nov. andPhaeoacremoniumSpecies Associated with Petri Disease on Grapevine Propagation Material and Young Grapevines in Algeria
Author(s) -
Wassila Aigoun-Mouhous,
Aleš Eichmeier,
Josep Armengol,
David Gramaje,
Maela León,
C. Chaouia,
Abdelghani Zitouni,
Alla Eddine Mahamedi,
Kateřina Baránková,
Akila Berraf-Tebbal
Publication year - 2021
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pdis-11-20-2380-re
Subject(s) - biology , internal transcribed spacer , botany , taxonomy (biology) , phylogenetic tree , cutting , genetics , gene
A field survey conducted on asymptomatic grapevine propagation material from nurseries and symptomatic young grapevines throughout different regions of Algeria yielded a collection of 70 Phaeoacremonium-like isolates and three Cadophora-like isolates. Based on morphology and DNA sequence data of β-tubulin (tub2) and actin, five Phaeoacremonium species were identified including Phaeoacremonium minimum (22 isolates), Phaeoacremonium venezuelense (19 isolates), Phaeoacremonium parasiticum (17 isolates), Phaeoacremonium australiense (8 isolates), and Phaeoacremonium iranianum (4 isolates). The latter two species (P. australiense and P. iranianum) were reported for the first time in Algeria. Multilocus phylogenetic analyses (internal transcribed spacer, tub2, and translation elongation factor 1-α) and morphological features, allowed the description of the three isolates belonging to the genus Cadophora (WAMC34, WAMC117, and WAMC118) as a novel species, named Cadophora sabaouae sp. nov. Pathogenicity tests were conducted on grapevine cuttings cultivar Cardinal. All the identified species were pathogenic on grapevine cuttings.