Open AccessEffectiveness of an Attenuated Zucchini yellow mosaic virus Isolate for Cross-Protecting Cucumber
Author(s) -
Yoshitaka Kosaka,
Bo-Song Ryang,
Takashi Kobori,
Hiroshi Shiomi,
Hisao Yasuhara,
Mitsunobu Kataoka
Publication year - 2006
Publication title -
plant disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.663
H-Index - 108
eISSN - 1943-7692
pISSN - 0191-2917
DOI - 10.1094/pd-90-0067
Subject(s) - zucchini yellow mosaic virus , biology , cucumber mosaic virus , papaya ringspot virus , inoculation , cucumovirus , horticulture , botany , virology , potyvirus , plant virus , virus
To cross-protect cucumber plants from Zucchini yellow mosaic virus (ZYMV), we used cold treatment to obtain an attenuated isolate of ZYMV, designated ZYMV-2002. ZYMV-2002 was obtained from a virulent ZYMV isolate after repeated low temperature treatment at 12.5 to 15°C followed by five cycles of single-plant transfer. The isolate produced very mild or no symptoms on cucurbit plants. In addition, inoculated cucumber plants had very similar fruit productivity to healthy control plants under field conditions. During field experiments in 2002 and 2003, when other viruses were also present, protected plants significantly suppressed infection with ZYMV, progression of disease severity, and reduction of fruit yield and quality. These results demonstrate that ZYMV-2002 is a potentially useful attenuated ZYMV isolate for reducing the impact of ZYMV.