Open AccessThe avrRxo1 Gene from the Rice Pathogen Xanthomonas oryzae pv. oryzicola Confers a Nonhost Defense Reaction on Maize with Resistance Gene Rxo1
Author(s) -
Bingyu Zhao,
Edna Ardales,
Anabela Raymundo,
Jianfa Bai,
Harold N. Trick,
Jan E. Leach,
Scot H. Hulbert
Publication year - 2004
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2004.17.7.771
Subject(s) - xanthomonas oryzae , xanthomonas oryzae pv. oryzae , biology , pathogen , gene , xanthomonas , effector , microbiology and biotechnology , gene expression , hypersensitive response , plant disease resistance , r gene , mutant , genetics , botany
Maize lines that contain the single dominant gene Rxo1 exhibit a rapid hypersensitive response (HR) after infiltration with the rice bacterial streak pathogen Xanthomonas oryzae pv. oryzicola, but not with the rice bacterial blight pathogen X. oryzae pv. oryzae. The avirulence effector gene that corresponds to Rxo1, designated avrRxo1, was identified in an X. oryzae pv. oryzicola genomic library. When introduced into X. oryzae pv. oryzae, clones containing avrRxo1 induced an HR on maize with Rxo1, but not on maize without Rxo1. The avrRxo1 gene is 1,266 bp long and shows no significant homology to any database sequences. When expressed in an X. oryzae pv. oryzae hrpC mutant that is deficient in the type III secretion system, avrRxo1 did not elicit the HR, indicating that the avrRxo1-Rxo1 interaction is dependent on type III secretion. Transient expression of avrRxo1 in onion cells after biolistic delivery revealed that the protein product was associated with the plasma membrane. Transient expression in maize lines carrying Rxo1 resulted in cell death, suggesting that AvrRxo1 functions from inside maize cells to elicit Rxo1-dependent pathogen recognition.