Open AccessPPI1: A Novel Pathogen-Induced Basic Region-Leucine Zipper (bZIP) Transcription Factor from Pepper
Author(s) -
Sang Jik Lee,
Mi Yeon Lee,
So Young Yi,
Sang Keun Oh,
Soon Ho Choi,
Nam Han Her,
Doil Choi,
Byung Whan Min,
Seung Gyun Yang,
Chee Hark Harn
Publication year - 2002
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2002.15.6.540
Subject(s) - bzip domain , leucine zipper , biology , atf3 , transcription factor , gene , basic helix loop helix leucine zipper transcription factors , complementary dna , genetics , dna binding domain , pepper , activating transcription factor 2 , activating transcription factor , microbiology and biotechnology , dna binding protein , gene expression , promoter , horticulture
We have isolated a full-length cDNA, PPI1 (pepper-PMMV interaction 1), encoding a novel basic region-leucine zipper (bZIP) DNA-binding protein, from expressed sequence tags differentially expressed in Capsicum chinense PI257284 infected with Pepper mild mottle virus (PMMV). PPI1 encodes a predicted protein of 170 amino acids and contains a putative DNA-binding domain that shares significant amino acid identity with ACGT-binding domains of members of the bZIP DNA-binding protein family. PPI1 was localized in the nucleus and had transcriptional activation activity in yeast. Transcripts of the PPI1 gene were preferentially induced during an incompatible interaction by inoculation with PMMV, Pseudomonas syringae pv. syringae 61, and Xanthomonas campestris pv. vesicatoria race 3. However, the PPI1 gene was not induced by abiotic stressors that activate the plant defense-signaling pathway. Our data provide the first evidence that a bZIP transcription factor is preferentially induced by pathogen attack, suggesting that PPI1 may play a specific functional role in the regulation of expression of plant defense-related genes.