Open AccessGene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus
Author(s) -
van Wezel R,
Liu H,
Po Tien,
John Stanley,
Yiguo Hong
Publication year - 2001
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2001.14.9.1125
Subject(s) - nicotiana benthamiana , green fluorescent protein , potato virus x , biology , virology , tomato yellow leaf curl virus , virus , nuclear localization sequence , plant virus , mcherry , microbiology and biotechnology , gene , nucleus , genetics
Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.