Mutants of Tobacco mosaic virus with Temperature-Sensitive Coat Proteins Induce Heat Shock Response in Tobacco Leaves | Zendy

Harald Jockusch | Zendy; Christiane Wiegand | Zendy; Birgit Mersch | Zendy; Daniela Rajes | Zendy

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Mutants of Tobacco mosaic virus with Temperature-Sensitive Coat Proteins Induce Heat Shock Response in Tobacco Leaves

Author(s) -

Harald Jockusch,

Christiane Wiegand,

Birgit Mersch,

Daniela Rajes

Publication year - 2001

Publication title -

molecular plant-microbe interactions

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.565

H-Index - 153

eISSN - 1943-7706

pISSN - 0894-0282

DOI - 10.1094/mpmi.2001.14.7.914

Subject(s) - tobacco mosaic virus , heat shock protein , mutant , coat protein , hsp70 , tobamovirus , biology , coat , wild type , virus , tobacco leaf , microbiology and biotechnology , virology , chemistry , biochemistry , rna , gene , paleontology , agricultural engineering , engineering

We analyzed, with respect to heat shock proteins (HSPs), systemically reacting tobacco leaves inoculated with Tobacco mosaic virus (TMV), wild-type vulgare, and temperature-sensitive coat protein (CP) mutants Ni 118 (P20L) and flavum (D19A), kept at 23 or 30°C. HSP18 and HSP70 mRNAs and proteins were induced with temperature-sensitive CP mutants after 1 to 2 days at 30°C. After 4 to 6 days, HSP70 was also induced at 23°C. The induction of HSPs paralleled the amount of insoluble TMV CP in leaf extracts, indicating that denatured TMV CP by itself induces a heat-shock response.

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