Open AccessMutants of Tobacco mosaic virus with Temperature-Sensitive Coat Proteins Induce Heat Shock Response in Tobacco Leaves
Author(s) -
Harald Jockusch,
Christiane Wiegand,
Birgit Mersch,
Daniela Rajes
Publication year - 2001
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2001.14.7.914
Subject(s) - tobacco mosaic virus , heat shock protein , mutant , coat protein , hsp70 , tobamovirus , biology , coat , wild type , virus , tobacco leaf , microbiology and biotechnology , virology , chemistry , biochemistry , rna , gene , paleontology , agricultural engineering , engineering
We analyzed, with respect to heat shock proteins (HSPs), systemically reacting tobacco leaves inoculated with Tobacco mosaic virus (TMV), wild-type vulgare, and temperature-sensitive coat protein (CP) mutants Ni 118 (P20L) and flavum (D19A), kept at 23 or 30°C. HSP18 and HSP70 mRNAs and proteins were induced with temperature-sensitive CP mutants after 1 to 2 days at 30°C. After 4 to 6 days, HSP70 was also induced at 23°C. The induction of HSPs paralleled the amount of insoluble TMV CP in leaf extracts, indicating that denatured TMV CP by itself induces a heat-shock response.